zebrafish methods and protocols

Download Free Zebrafish: Methods and Protocols

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Optimized CRISPR–Cas9 System for Genome Editing in

This protocol describes how to generate and genotype mutants using an optimized CRISPR–Cas9 genome-editing system in zebrafish (CRISPRscan) Because single guide RNAs (sgRNAs) have variable efficiency when targeting specific loci our protocol starts by explaining how to use the web tool CRISPRscan to design highly efficient sgRNAs The CRISPRscan algorithm is based on the results of

ZebraFISH: fluorescent in situ hybridization protocol and

We present a method and protocol for fluorescent in situ hybridization (FISH) in zebrafish embryos to enable three-dimensional imaging of patterns of gene expression using confocal laser scanning microscopy We describe the development of our protocol and the processing workflow of the three-dimensional images from the confocal microscope We refer to this protocol as zebraFISH FISH is

Zebrafish

Zebrafish: Methods and Protocols Second Edition guides readers through methods for mutagenesis and genome editing in zebrafish applications of GFP-expressing transgenic fish techniques for cancer models imaging of infection and host-pathogen interactions metabolism and transport of lipids and the structure and function of neural circuits and their role in generating behavior

[PDF] Essential Zebrafish Methods Genetics And Genomics

Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish The methods in this volume were hand-selected by the editors whose goal was to a provide a handy and

Methods and Protocols

Methods and Protocols Delivery into Embryos The journal genesis: the Journal of Genetics and Development devoted an entire issue to the use of Morpholino oligos The nice folks at Wiley have made this entire journal issue open-access In this issue you will find delivery methods and results for zebrafish Xenopus chick and mouse embryos and you will also find the first use of splice

From the sea to the bench: Marine microeukaryote

Most molecular and cell biology research is performed on just a few model organisms The most commonly studied organisms are humans mice yeast worms the zebrafish the plant Arabidopsis and the fruit fly Focusing on this handful of models has been meaningful and productive strategy for understanding organisms in terrestrial and freshwater environments – and during a time when

Highly Efficient CRISPR

The methods described here provide a simple and efficient method for inducing deletion mutations Injection of zebrafish eggs with pairs of dgRNPs can be used to produce F0 embryos with substantial amounts of tissue composed of cells bi-allelic for newly induced deletions These genetically mosaic F0 embryos can be useful especially as part of

Overview Of Methods For Parthenogenesis

Zebrafish eggs are fertilized outside the body making it possible to manipulate the process to advantage For example haploid embryos can be produced by fertilizing eggs with sperm that have been irradiated with ultra violet light (UV sperm) destroying the sperm DNA so that it cannot contribute genetically to the embryo but leaving the sperm intact and able to activate the eggs Haploid

Zebrafish: Methods and Protocols / Edition 1 by Graham

In the last 20 years research activity using the zebrafish Danio rerio has increased dramatically due in part to the ease of breeding and raising them their genetic tractability embryonic accessibility and their imaging potential In Zebrafish#58 Methods and Prools expert

Zebrafish Larviculture

Protocols for zebrafish larviculture must incorporate an understanding of (1) the natural history biology behavior and nutritional requirements of the developing fish and (2) the relationship between these traits and the physical and chemical properties of water The best and most effective larval rearing techniques strike the proper balance between the two and require vigilant attentive

Zebrafish Protocols for Neurobehavioral Research :

Zebrafish Protocols for Neurobehavioral Research addresses protocols for both larval and adult models written by the leading experts in the field of zebrafish research Part I of this book TongWeis advantage of the high-throughput nature of larval models to offer protocols for research requiring high output easily manipulated screens The second half of the book focuses on the robust and

Zebrafish: Methods and Protocols 2nd Edition

Zebrafish: Methods and Protocols Second Edition guides readers through methods for mutagenesis and genome editing in zebrafish applications of GFP-expressing transgenic fish techniques for cancer models imaging of infection and host-pathogen interactions metabolism and transport of lipids and the structure and function of neural circuits and their role in generating behavior Written in

Expansion microscopy of zebrafish for neuroscience and

We explore the utility of expansion microscopy (ExM) in neuroscience and developmental biology using the zebrafish model Regarding neuroscience studies ExM enables the tracing of cellular processes in the zebrafish brain as well as the imaging of synapses and their biomolecular content and organization Regarding development ExM enables the resolving of nuclear compartments

Scientific Protocols

Bromodeoxyuridine (BrdU) labeling and immunohistochemical detection in adult zebrafish brain Fish Neuroscience Model Organisms scientificprotocols authored over 5 years ago Protocol Discussion Authors: Ayca Arslan Ergul Dilara zge Halim Michelle M Adams Abstract Zebrafish is an ideal model organism to study adult neurogenesis New neurons are born in various parts of zebrafish

Highly Efficient CRISPR

The methods described here provide a simple and efficient method for inducing deletion mutations Injection of zebrafish eggs with pairs of dgRNPs can be used to produce F0 embryos with substantial amounts of tissue composed of cells bi-allelic for newly induced deletions These genetically mosaic F0 embryos can be useful especially as part of

[PDF] Essential Zebrafish Methods Cell And

Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish The methods in this volume were hand-selected by the editors whose goal was to a provide a handy and

Ultrastructural Mapping of the Zebrafish

2 1 Zebrafish Animal Model Zebrafish (wild-type Danio rerio) were maintained at 28C in a 13 h light and 11 h dark cycle Embryos were collected by natural spawning and raised at 28 5C in E3 solution according to standard protocols [] Note that from previous organogenesis studies of the ZF digestive system it was determined that from 6 days after fertilisation (dpf) when the yolk is

Behavioural assay that measures the startle response in

The startle response in fish is a pattern of rapid contractions of the axial musculature used for swimming elicited by external stimuli In zebrafish (Danio rerio) it has long been employed for analyses of motor function sensory physiology and basic forms of learning (e g Kimmel et al 1974)

Zebrafish

In Zebrafish: Methods and Protocols expert researchers provide a collection of experimental techniques for the genetic modification manipulation and in vivo live imaging of zebrafish This volume collects sophisticated and practical techniques including several not yet widely known from laboratories around the world Written in the highly successful Methods in Molecular Biology™ series

Developing a zebrafish model of neurovascular coupling

Developing a zebrafish model of neurovascular coupling (NVC) Overview Publications Project background When a brain region becomes active blood flow to the area is increased to ensure cells receive an adequate supply of glucose and oxygen from the surrounding vasculature This is controlled by complex interactions between the cells of the nervous system and blood vessels a process termed

Proteomics Analysis of Early Developmental Stages of

Comparison of the efficiencies of deyolking protocols: reference [16] versus the new one (A) Protein concentration obtained using protocols in 1-step versions (left chart) and in full versions (right chart) (B) SDS-PAGE of proteins extracted from zebrafish embryos at 1-cell stage (left panel) and high stage (right panel) using the reference protocols versus new protocols

Download [PDF] Essential Zebrafish Methods Cell And

Download Essential Zebrafish Methods Cell And Developmental Biology ebook PDF or Read Online books in PDF EPUB and Mobi Format Provides busy researchers a quick reference for time-tested methods and protocols that really work updated where possible by the original authors Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and

A glitch in the Matrix: Using virtual reality to

A new virtual reality system allows us to manipulate the sensory environment of adult zebrafish at will while simultaneously analyzing neural activity This approach can be used to explore how the brain processes complex sensory inputs and how predictions control behaviors

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