3t3-l1 adipocytes promote the growth of mammary

3T3

Murine mammary epithelium grows in association with predominantly adipocyte stroma in vivo To investigate potential growth-promoting effects of adipocytes on mammary epithelium we developed a co-culture system of mammary epithelium and adipocytes by taking advantage of the 3T3-L1 cell line

Cyanidine

Aronia melamocarpa (AM) is a rich source of anthocyanins which are known to help prevent obesity The cyanidine-3-O-galactoside enriched AM extract (AM-Ex) containing more cyanidine-3-O-galactoside than conventional AM extract was recently developed The objective of this study was to examine the effect of AM-Ex on adipogenesis and its action mechanisms in vitro using 3T3-L1 adipocytes

3T3

Murine mammary epithelium grows in association with predominantly adipocyte stroma in vivo To investigate potential growth-promoting effects of adipocytes on mammary epithelium we developed a co-culture system of mammary epithelium and adipocytes by taking advantage of the 3T3-L1 cell line

The Mechanism of Phillyrin from the Leaves of Forsythia

FEA could promote the glucose upTongWei of IR 3T3-L1 adipocytes at the concentrations of 200 100 (P0 01) and 50 μM (P0 5) (Figure 2(b)) The glucose upTongWei of IR 3T3-L1 adipocytes was promoted by RFG at the concentrations of 100 μM (P0 5) but the

Anti

9/30/2016The levels of intracellular lipid accumulation were visualized by ORO staining on day 6 Lipid accumulations of 3T3-L1 adipocytes treated with 50 μg/mL CZE during a b and c stages were significantly inhibited by 62 0% 85 9% and 55 2% respectively However we did not observe a significant difference between CZE-treated adipocytes and

Volufi line

adipocytes First a pool of competent pre-adipocytes is formed The cells divide actively and at the end of the growth phase enter a differentiation phase in order to acquire all the functions of a mature adipocyte Mammary ridge Mammary gland Cutaneous and fatty tissue Lactiferous sinus Pectoral muscles Ribs Intercostal muscles

Frontiers

9/19/2017Figure 1 Cross-talk between adipocytes macrophages and cancer cells modulating the tumor microenvironment In the protumorigenic state macrophages are attracted to the tumor site via chemokines such as CCL-2 -4 and -5 produced by malignant cells resident macrophages and inflamed adipose tissue Obese adipose tissue can favor tumor growth and development by producing anti-

Establishment of a Mammary Stromal Fibroblastic Cell Line

Mammary stromal adipose tissue remodeling is important for appropriate mammary gland development during pregnancy lactation and involution However the precise mechanisms underlying mammary stromal adipose tissue remodeling remain unclear We have established a mammary stromal fibroblastlike cell line (MSF) from primary mouse mammary culture by introducing a temperature

Matthew Brady

Matthew Brady's profile publications research topics and co-authors Keywords MN Johnson Singh P Patel FN McClintock MK Brady MJ Conzen SD Mammary Adipose Tissue-Derived Lysophospholipids Promote Estrogen Receptor-Negative Mammary Epithelial Cell Proliferation Cancer Prev Res (Phila) 2016 05 9(5):367-78 PMID: 26862086 View in: PubMed Singh P Brock CO

THE JOURNAL OF BIOLOGICAL CHEMISTRY 2002 by The

ing 3T3-L1 cells was unable to promote protein kinase B phosphorylation after the addition of recombinant IG-FBP-4 Thus we demonstrated that Wnt-1 induces ex-pression of antiapoptotic genes in 3T3-L1 preadipocytes such as IGF-I and IGF-II which allows these cells to resist apoptosis in response to serum deprivation

Dietary fat increases solid tumor growth and metastasis of

Subsequently 4T1 mammary carcinoma cells were injected into the inguinal mammary fat pads of mice fed continuously on their respective diets Cell-cycle progression angiogenesis and immune cells in tumor tissues proteases and adhesion molecules in the lungs and serum cytokine levels were analyzed with immunohistochemistry Western blotting and enzyme-linked immunosorbent assay (ELISA

Obestatin: A new metabolic player in the pancreas and

Obestatin exerts proliferative and antiapoptotic effects in 3T3‐L1 adipocytes through activation of PI3K/Akt and ERK1/2 pathways 10 38 (Fig 2) Furthermore it promotes differentiation of both 3T3‐L1 adipocytes and human SC and OM adipocytes and increases adipogenic gene expression

IYENGAR_RESPONSE_TO_ADIPOCYTE_FACTORS

Genes up-regulated in MCF-7 cells (breast cancer) in response to growth medium from L3T3-L1 cells (differentiated to pre-adipocytes) Full description or abstract: Mammary epithelial cells are embedded in a unique extracellular environment to which adipocytes and other stromal cells contribute

Molecular links between obesity and breast cancer in

Murine 3T3-L1 adipocytes are a useful and widely used model system however 3T3-L1 adipocytes may not secrete the same array of adipokines in the same concentrations as human adipocytes from an obese individual Human adipocyte cell lines from obese and slim individuals very easily established in culture from liposuction samples could provide a useful alternative to murine adipocyte cell

Molecular mechanism of 1 25

3T3-L1 cell differentiation into adipocytes in a dose-dependent man-ner however the inhibition is ineffective 24–48 h after the differen-tiation is initiated suggesting that 1 25(OH) 2D 3 inhibits only the early events of the adipogenic program Treatment of 3T3-L1 cells with 1 25(OH) 2D 3 does not block the mitotic clonal expansion or C/EBP induction rather 1 25(OH) 2D 3

CCAAT/enhancer

Hormonal induction of growth-arrested 3T3-L1 preadipocytes triggers a signaling cascade that culminates in adipogenesis CCAAT/enhancer-binding protein (C/EBP)β is expressed immediately but gains DNA-binding activity only after a long lag as the cells synchronously begin mitotic clonal expansion (MCE) After MCE a process required for adipogenesis C/EBPβ activates expression of C/EBPα and

Cell

5/1/1985Mammary epithelium from mid-pregnant mice was plated on confluent irradiated monolayers of 3T3-L1 cells a subclone of the Swiss 3T3 cell line that differentiates into adipocytes in monolayer culture and other cell monolayers (3T3-C2 cells Swiss 3T3 cells and human foreskin fibroblasts)

Cyanidine

Aronia melamocarpa (AM) is a rich source of anthocyanins which are known to help prevent obesity The cyanidine-3-O-galactoside enriched AM extract (AM-Ex) containing more cyanidine-3-O-galactoside than conventional AM extract was recently developed The objective of this study was to examine the effect of AM-Ex on adipogenesis and its action mechanisms in vitro using 3T3-L1 adipocytes

Adipose Cells

Here the authors report that laminin-411 is the specific isoform of laminin synthesized in adipocytes Reverse transcription-polymerase chain reaction (RT-PCR) of mRNA from mouse 3T3-L1 cells yielded amplified fragments only for α4β1γ1 Northern blots showed that the levels of α4 β1 and γ1 mRNAs increased 2 5-fold during adipose

Cyanidine

Aronia melamocarpa (AM) is a rich source of anthocyanins which are known to help prevent obesity The cyanidine-3-O-galactoside enriched AM extract (AM-Ex) containing more cyanidine-3-O-galactoside than conventional AM extract was recently developed The objective of this study was to examine the effect of AM-Ex on adipogenesis and its action mechanisms in vitro using 3T3-L1 adipocytes

Control of breast cancer cell growth by adipocyte

Obesity is one of the most challenging and growing health problems worldwide Epidemiologic studies now provide compelling evidence that obesity is a risk factor for both cancer incidence and mortality In particular obesity increases rates of breast cancer in postmenopausal women and is associated with poorer survival and increased recurrence of disease regardless the menopausal status

3T3

3T3-L1 adipocytes promote the growth of mammary epithelium (Q42798770) From Wikidata Jump to navigation Jump to search scientific article published on March 1984 edit Language Label Description Also known as English: 3T3-L1 adipocytes promote the growth of mammary epithelium scientific article published on March 1984 Statements instance of scholarly article 1 reference stated in

Branched chain amino acids alter fatty acid profile in

Branched chain amino acids (BCAAs) are important substrates for milk protein synthesis in the mammary gland and are tightly related to lipid metabolism No study has been performed examining the role of BCAAs with high fat diets on milk fat synthesis This study was designed to investigate the effect of dietary BCAAs on growth performance of piglets progeny body weight and milk fat

Conversion of non

Non-adipogenic fibroblasts were induced into adipocytes by treatment with a defined hormone mixture including epidermal growth factor hepatocyte growth factor dexamethasone and insulin Non-adipogenic fibroblasts were directly converted into adipocytes without a pre-adipocyte stage

3T3

Experimental Cell Research 151 (1984) 112-122 3T3-L1 Adipocytes Promote the Growth of Mammary Epithelium JEDD F LEVINE* and FRANK E STOCKDALE Division of Oncology Department of Medicine Room S-025 Stanford University School of Medicine Stanford CA 94305 USA Murine mammary epithelium grows in association with predominantly adipocyte stroma in vivo

Support: Cell Manuals

3T3-L1 adipocytes have been fundamental in metabolic disease research for 30 years Originally derived from Swiss mouse embryo tissue by Dr Ho ward Green of Harvard Medical School the 3T3-L1 system has been pivotal in advancing the underst anding of basic cellular mechanisms associated with diabetes obesity and related disorders

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